Coding

Part:BBa_K1393003:Design

Designed by: Jiajun Tan   Group: iGEM14_HUST-China   (2014-10-10)


outer membrane porin protein C from Escherichia coli BL21(DE3)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In general, the amino acid sequences of the external loops are less conservative, and thus these loops may be relatively tolerant to insertion and deletion. We used the external loop as the point of insertion for foreign peptides (copper binding polypeptide or polyhistidine) for cell surface display.


Source

This part is from genome of Escherichia coli BL21(DE3).

References